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    Several places in the Philippines including Samar and Southern Leyte, have reported 50 acute diarrhea cases due to E. coli contamination. It is transmitted to humans partly through contaminated food consumption. It includes drinking water with fecal contamination according to DOH (2022). The same case with Staphylococcus aureus, which is a major bacterial human pathogen that causes a wide variety of clinical manifestations. Treatment remains challenging to manage due to the emergence of multi-drug resistant strains such as MRSA (Methicillin-Resistant Staphylococcus aureus) (2022). This prompted the researchers to explore the phytochemical properties of Devils Ivy Pothos and Noni leaves extract and to study its antibacterial activity with different types of bacteria. 

    Devils Ivy Pothos and Noni leave extract were examined at DOST and the results revealed that these plants contain Sterols, Flavonoids, Alkaloids, Glycosides, Tannins, and Saponins, except for Triterpenes. Three treatments were formulated to test the zone of inhibition and reactivity against Escherichia coli, Staphylococcus aureus, and Salmonella typhimurium. Treatment 1 containing 75% Pothos and 25% Noni leaf extract and Treatment 3 containing 50% Pothos and 50% Noni Leaf extract produced complete inhibitory activity (+++) with mild reactivity against the three tested microorganisms. Furthermore, Treatment 2 containing 25% Pothos and 75% Noni Leaf extract produced complete inhibitory (+++) with mild reactivity against Escherichia coli and Salmonella typhimurium, while on Staphylococcus Aureus, it only produced partial inhibitory activity (+++) with mild reactivity. 

    The result of the T-Test: Paired Two Sample for Means revealed that treatment one and positive control have a significant difference. Therefore, the null hypothesis is rejected. In general, the results of this study showed that all treatments of Devils Ivy Pothos (Epipremnum aureum) and Noni (Morinda citrifolia) leaves extract were less effective compared to positive control but it also possesses antimicrobial property.